Fig. 4

Growth-independent amorphadiene production. a Schematic overview of the strain tested. Blue arrows show the endogenous MEP pathway, while the orange ones the orthogonal MVA pathway. In green arrows, the PHB biosynthetic pathway is depicted. Red cross represent genes deletion of either dxr (MEP pathway) or phaB (PHB pathway) genes. C₁₅H₂₄ is the brute formula of the reporter molecule amorphadiene. b–e Monitoring of b OD₆₀₀, c glucose concentration, d pH, e amorphadiene concentration. f volumetric productivities of PHB and organic acids (PYR: pyruvate; 2-OXO: 2-oxoglutarate). Determination of g amorphadiene/biomass ratios, h amorphadiene volumetric productivity and i amorphadiene yield on glucose