Growth-uncoupled isoprenoid synthesis in Rhodobacter sphaeroides

Table 3 Titers and yields obtained from shaking flasks experiments of strains Rs265 and Rs265-MVA

Plasmid	Condition	Genomic background	Amorphadiene titers, C_P (mg · L⁻¹)		Amorphadiene yield on glucose, Y_P/S (mg · g⁻¹)		Final Biomass concentration, C_X (g · L⁻¹)		Amorphadiene on Biomass, Y_P/X (mg · g⁻¹)
Plasmid	Condition	Genomic background	Value	St. dev.	Value	St. dev.	Value	St. dev.	Value	St. dev.
pBBR-ads	Nitrogen excess	wt	4.4	0.3	1.41	0.15	1.533	0.062	2.9	0.2
		ΔphaC1 ΔphaC2	3.3	0.1	ND	ND	1.491	0.000	2.2	0.1
		ΔphaB	4.7	0.2	1.49	0.07	1.724	0.110	2.7	0.1
	Nitrogen limited	wt	2.4	0.2	0.73	0.05	0.805	0.122	2.9	0.2
		ΔphaC1 ΔphaC2	1.4	0.2	ND.	ND.	0.693	0.034	2.0	0.3
		ΔphaB	2.2	0.2	0.69	0.04	0.693	0.112	3.2	0.2
pBBR-MVA-ads	Nitrogen excess	wt	41.8	1.8	13.64	0.56	1.289	0.019	32.4	1.4
		ΔphaC1 ΔphaC2	39.3	4.4	ND.	ND.	1.060	0.856	37.1	4.2
		ΔphaB	56.4	2.1	18.25	0.68	1.466	0.036	38.5	1.5
	Nitrogen limited	wt	23.6	2.3	7.65	0.76	0.656	0.115	35.9	3.6
		ΔphaC1 ΔphaC2	24.1	2.1	ND.	ND.	0.539	0.260	44.8	3.9
		ΔphaB	34.4	2.2	11.38	0.73	0.539	0.022	63.7	4.0
pBBR-MVA-ads	Nitrogen excess	MVAint_Δdxr	15.6	3.9	3.41	0.23	2.229	0.144	7.0	1.7
	Nitrogen excess	MVAint_ΔdxrΔphaB	28.0	1.0	9.67	0.86	2.187	0.080	12.8	0.5
	Nitrogen limited	MVAint_Δdxr	13.5	1.9	3.80	0.82	0.597	0.142	22.6	3.1
	Nitrogen limited	MVAint_ΔdxrΔphaB	19.5	0.8	5.65	0.29	0.528	0.161	36.9	1.6

Data were obtained from end-point measurements under nitrogen excess and nitrogen-limited conditions
ND not determined

ISSN: 2731-3654