Fig. 2

Hydrocarbon production of E. coli strain BL21(DE3)∆∆ containing engineered a ADO- and b CvFAP-dependant routes. Cultures (10 mL) were grown in LB medium containing 30 μg/mL kanamycin and 50 µg/mL chloramphenicol (pTrF/TrADO and pPrF/TrADO only) for 4–6 h at 37 °C and 180 rpm. Protein induction (100 μM IPTG) was performed for trc-containing constructs, and triplicate aliquots (1 mL) each of 3 biological replicate cultures were sealed into glass vials (3 mL) at 30 °C for 16–18 h at 200 rpm. For CvFAP-dependent constructs, the cultures were illuminated continuously with a blue LED (455 nm or 470 nm). Gaseous hydrocarbon levels were determined by manual headspace injection on an Agilent 490 Micro GC. Errors represent one standard deviation of the replicates (biological and/or technical triplicates). Constructs (inset): ProD = pPrAFYSCIB; ProD/ProD = pPrAFYSCPrIB; Trc = pTrAFYSCIB; Trc/Trc = pTrAFYSCTrIB and Trc-lacIq = pTrΔLAFYSCIB