Fig. 4

Rescue of kcn11 phenotypes by elevation of osmolarity via addition of sucrose. a WT or kcn11 cells were treated with different concentrations of sucrose for 1 day after depletion of nitrogen. Bar = 5 μm. b Cell growth of WT or kcn11 cells in N-depleted media for 1 day with addition of different concentrations of sucrose. c WT or kcn11 cells were grown in N-depleted media for 2 days in the presence or absence of 40 mM sucrose. d–f WT and kcn11 cells were grown in N-depleted media for 2 days with or without 40 mM sucrose followed by Nile Red staining (d), by measuring cellular number (e) and size (f) of the lipid droplets. Bar = 5 µm. Data shown are mean ± sem (n = 50). N.S., no significant. ***P < 0.0001 (t test)