Fig. 6

Overexpression of ace3-L improves protein production in an industrial strain. a SDS-PAGE of culture supernatants from T. reesei parental strain T4abc and its daughter strain LT83 (expressing ace3-L). An equal volume of culture supernatant was loaded in each lane. The total secreted protein concentration of each strain (as measured by Bradford assays) was shown as relative ratio to that of strain T4abc grown glucose/sophorose (set at 1). Glu/Sop, glucose/sophorose; Glu, glucose; M, protein molecular weight marker. b Protein production of T. reesei parental strain T4abc and daughter strain LT83 in 2-L fermenters. The total protein produced by the parental strain fed with glucose/sophorose at the end of fermentation was arbitrarily set at 1, and the relative amounts of protein produced by each strain at each time points were plotted