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Table 2 Saccharification parameters of the different S. cerevisiae strains displaying hemicellulolytic enzymes

From: Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

Substrate

Potential xylose (mM)

Inoculum

Strain

Xylosef (mM)

Xylitolf (mM)

Saccharification yield (%)

Xylan from beechwood

68.1

140 U of xylanase activity/L

ER-X

7.85 ± 0.40

9.72 ± 0.26

25.8 ± 1.0

PE-2-X

17.1 ± 0.1

9.98 ± 0.39

39.8 ± 0.4

CAT-1-X

11.4 ± 0.2

6.60 ± 0.51

26.4 ± 1.1

CA11-X

14.5 ± 0.1

14.1 ± 0.8

42.1 ± 1.1

Liquor 29XPot (S0 = 3.67 and LSR = 8 g/g)

184

140 U of xylanase activity/L

ER-X

58.5 ± 0.6

16.5 ± 0.5

40.8 ± 0.6

PE-2-X

78.2 ± 0.0

11.5 ± 0.3

48.7 ± 0.2

CAT-1-X

62.9 ± 0.2

9.94 ± 0.14

39.6 ± 0.2

CA11-X

67.8 ± 3.2

22.9 ± 0.6

49.3 ± 2.1

10 g of fresh yeast/L

ER-X

64.6 ± 1.6

24.4 ± 1.1

38.9 ± 1.6

PE-2-X

96.7 ± 5.9

13.8 ± 0.9

49.1 ± 1.6

CAT-1-X

81.1 ± 0.1

14.2 ± 0.1

44.5 ± 3.0

CA11-X

86.4 ± 3.0

23.4 ± 0.7

45.5 ± 1.6

  1. Saccharification assays were performed in beechwood xylan or corn cob liquor 29XPot at 40 °C. Data represents the average ± SD from two biological replicates
  2. S0: severity; LSR: liquid-solid ratio