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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq

Fig. 6

Cell growth of recombinant and wild-type strains of Z. mobilis containing the control plasmid pEZ15A and plasmid constructs of pET-Tc3 and pET-Tc5 (M) at pH 3.6, 4.0, and 6.0 without tetracycline (a, c) and with 0.8 μg/mL tetracycline induction (b, d). pEZ-Tc3, plasmid construct expressing operon ZMO0956–ZMO0958 encoding cytochrome bc1 complex; pEZ-Tc5 (M), plasmid construct expressing operon ZMO1428–ZMO1432 encoding RND efflux system with a mutation in ZMO1432. At least two independent experiments were performed with similar results. Values are the mean of one representative experiment with three technical replicates. Error bars represent standard deviations

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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