Skip to main content

Table 1 Strains constructed in this study

From: Studies on sugar transporter CRT1 reveal new characteristics that are critical for cellulase induction in Trichoderma reesei

Name Description/genotype
Aspergillus nidulans
 FGSC A4 WT strain
Neurospora crassa
 FGSC 9719 WT strain
Trichoderma reesei
 QM9414 Early producer strain with improved protein production
 M44 Mutated QM9414
 RUT-C30 Carbon catabolite derepressed mutant strain
 M44 \(\Delta\)crt1 M44 \(\Delta\)crt1
 trSS-1 M44 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))
 trSS-2 M44 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))
 trSS-6 M44 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))
 trSS-7 M44 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))
 trSS-8 M44 \(\Delta\)crt1 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))
 trSS-9 M44 \(\Delta\)crt1 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))
 trSS-12 M44 \(\Delta\)crt1 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))
 trSS-13 M44 \(\Delta\)crt1 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))
 trSS-14 QM9414 \(\Delta\)crt1
 trSS-15 QM9414 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))
 trSS-16 QM9414 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))
 trSS-18 QM9414 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))
 trSS-19 QM9414 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))
Saccharomyces cerevisiae
 FY834 MAT\(\upalpha\) his3\(\Delta\)200 ura3-52 leu2\(\Delta\)1 lys2\(\Delta\)202 trp1\(\Delta\)63
 AFY09 EBY.VW5000 mal31\(\Delta\)::loxP-kanMX4-loxP mal21\(\Delta\)::loxP-hphNT1-loxP
 CEN.PK2-1D MAT\(\upalpha\) ura3-52 his3-\(\Delta\)1 leu2-3,112 trp1-289 MAL2-8\(^{\mathrm{c}}\)
 ySS1 AFY09 URA3::NcGH1-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ADH1}}\), HIS3)
 ySS1 CRT1-RUT-C30 ySS1 crt1-RUT-C30 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 ySS1 CRT1-QM6a ySS1 crt1-QM6a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 ySS1 NcCDT-1 ySS1 NcCDT-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 ySS1 NcCDT-2 ySS1 NcCDT-2 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 ySS1 CEL1a ySS1 cel1a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 yJR33 CEN.PK2-1D URA3::NcGH1-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ADH1}}\), HIS3)
 yJR33 CRT1-RUT-C30 yJR33 crt1-RUT-C30 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 yJR33 CRT1-QM6a yJR33 crt1-QM6a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 yJR33 NcCDT-1 yJR33 NcCDT-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 yJR33 NcCDT-2 yJR33 NcCDT-2 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)
 yJR33 CEL1a yJR33 cel1a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)