Table 1 Strains constructed in this study

Aspergillus nidulans

 FGSC A4

WT strain

Neurospora crassa

 FGSC 9719

WT strain

Trichoderma reesei

 QM9414

Early producer strain with improved protein production

 M44

Mutated QM9414

 RUT-C30

Carbon catabolite derepressed mutant strain

 M44 \(\Delta\)crt1

M44 \(\Delta\)crt1

 trSS-1

M44 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))

 trSS-2

M44 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))

 trSS-6

M44 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))

 trSS-7

M44 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))

 trSS-8

M44 \(\Delta\)crt1 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))

 trSS-9

M44 \(\Delta\)crt1 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))

 trSS-12

M44 \(\Delta\)crt1 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))

 trSS-13

M44 \(\Delta\)crt1 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))

 trSS-14

QM9414 \(\Delta\)crt1

 trSS-15

QM9414 pep1::crt1-RUT-C30 (P\(_{\mathrm{pdc1}}\))

 trSS-16

QM9414 pep1::crt1-QM6a (P\(_{\mathrm{pdc1}}\))

 trSS-18

QM9414 pep1::AnLacpB (P\(_{\mathrm{pdc1}}\))

 trSS-19

QM9414 pep1::NcCDT-1 (P\(_{\mathrm{pdc1}}\))

Saccharomyces cerevisiae

 FY834

MAT\(\upalpha\) his3\(\Delta\)200 ura3-52 leu2\(\Delta\)1 lys2\(\Delta\)202 trp1\(\Delta\)63

 AFY09

EBY.VW5000 mal31\(\Delta\)::loxP-kanMX4-loxP mal21\(\Delta\)::loxP-hphNT1-loxP

 CEN.PK2-1D

MAT\(\upalpha\) ura3-52 his3-\(\Delta\)1 leu2-3,112 trp1-289 MAL2-8\(^{\mathrm{c}}\)

 ySS1

AFY09 URA3::NcGH1-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ADH1}}\), HIS3)

 ySS1 CRT1-RUT-C30

ySS1 crt1-RUT-C30 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 ySS1 CRT1-QM6a

ySS1 crt1-QM6a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 ySS1 NcCDT-1

ySS1 NcCDT-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 ySS1 NcCDT-2

ySS1 NcCDT-2 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 ySS1 CEL1a

ySS1 cel1a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 yJR33

CEN.PK2-1D URA3::NcGH1-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ADH1}}\), HIS3)

 yJR33 CRT1-RUT-C30

yJR33 crt1-RUT-C30 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 yJR33 CRT1-QM6a

yJR33 crt1-QM6a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 yJR33 NcCDT-1

yJR33 NcCDT-1 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 yJR33 NcCDT-2

yJR33 NcCDT-2 (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)

 yJR33 CEL1a

yJR33 cel1a (P\(_{\mathrm{PGK1}}\), T\(_{\mathrm{ENO1}}\), CEN6/ARS4, URA3)