Fig. 4

Testing sexual outcrossing in T. aurantiacus: a Sequencing data of the pyrE gene of two 5-FOA-resistant isolates (FOAR1-2) were aligned to the pyrE reference sequence (primers are listed in Table 2). FOAR2 was crossed with the hygromycin B-resistant strain taRG008 (hph strain). The progeny of this cross was analyzed through b PCR amplification of the hph gene (wild type, FOAR strains and taRG008 were included as controls). c Sequencing data of the pyrE gene sequence of the crossed strains in (b) were aligned to the native pyrE reference sequence. The sequence analysis was performed with Geneious version 11.1 (Biomatters). This analysis indicated that only the progeny isolates displayed genomic integrations of the hph gene and the pyrE mutation from FOAR2