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Table 2 Transcriptomics and q-PCR analysis of the expression of genes related to fatty acid synthesis

From: Low-temperature effects on docosahexaenoic acid biosynthesis in Schizochytrium sp. TIO01 and its proposed underlying mechanism

Name Description RNA-SEQ 16 °C vs 28 °C (log2Fold Change) Q-PCR 16 °C vs 28 °C (log2Fold Change)
Genes related to SFA synthesis
 ACACA Acetyl-CoA carboxylase − 0.99 − 1.14 ± 0.57
 FAS Fatty acid synthase − 1.52 − 4.02 ± 0.53
 ELO Long-chain fatty acid elongase − 1.08 − 2.43 ± 0.81
 VLCR Very long-chain 3-oxoacyl-CoA reductase − 1.07 − 0.91 ± 0.50
 ME Malic enzyme − 1.03 − 1.11 ± 0.62
Genes related to PUFA synthesis and the pentose phosphate pathway
 PfaA Polyketide synthase subunit A − 0.88 0.35 ± 0.22
 PfaB Polyketide synthase subunit B − 0.79 0.05 ± 0.29
 PfaC Polyketide synthase subunit C − 0.66 0.12 ± 0.38
 PGD Glucose-6-phosphate 1-dehydrogenase 1.72 3.78 ± 0.57
Genes related to FA metabolism in organelles
 ACADM-3 Acyl-CoA dehydrogenase − 1.39 − 2.36 ± 0.65
 ACADM-5 Acyl-CoA dehydrogenase − 1.11 − 1.80 ± 0.21
 ECHS-2 Enoyl-CoA hydratase 2.47 1.33 ± 0.22
 ECHS-6 Enoyl-CoA hydratase 1.54 1.42 ± 0.16
Genes related to glycolysis
 HK Glycolysis hexokinase − 1.05 − 0.62 ± 0.35
 PFKA 6-Phosphofructokinase − 1.48 − 0.98 ± 0.21
 PGAM Phosphoglycerate mutase − 1.22 0.14 ± 0.42
 PC Pyruvate carboxylase − 1.79 − 1.48 ± 0.031
Genes related to branched-chain amino acid degradation and the TCA cycle
 TAT Tyrosine aminotransferase 1.77 2.5 ± 0.2
 HGD Homogentisate 1,2-dioxygenase 1.46 1.08 ± 0.19
 FAH Fumarylacetoacetase 1.17 2.23 ± 0.63
 OXCT 3-Oxoacid CoA-transferase 1.06 1.12 ± 0.11
 ACCAT Acetyl-CoA C-acetyltransferase 1.58 2.62 ± 0.39
 BCATC-1 Ketol-acid reductoisomerase − 1.12 − 1.36 ± 0.41
 BCATC-2 Ketol-acid reductoisomerase 1.28 1.59 ± 0.27
 BCAT Branched-chain amino acid aminotransferase 1.26 2.69 ± 0.14
 BCKDH 2-Oxoisovalerate dehydrogenase E1 component 1.11 1.14 ± 0.07
 DBT 2-Oxoisovalerate dehydrogenase E2 component 1.52 3.46 ± 0.21
 GCDH Glutaryl-CoA dehydrogenase 1.07 0.52 ± 0.18
 LEU 3-Isopropylmalate dehydratase 2.49 3.08 ± 0.15
 IVD-2 Isovaleryl-CoA dehydrogenase 2.31 0.91 ± 0.28
 IVD-3 Isovaleryl-CoA dehydrogenase 1.66 2.64 ± 0.24
 MCCB 3-Methylcrotonyl-CoA carboxylase beta subunit 1.31 2.17 ± 0.27
 HMGCS Hydroxymethylglutaryl-CoA synthase − 1.19 − 2.18 ± 0.31
 LSC Succinyl-CoA synthetase − 2.72 − 1.3 ± 0.21