Table 2 Transcriptomics and q-PCR analysis of the expression of genes related to fatty acid synthesis
Name | Description | RNA-SEQ 16 °C vs 28 °C (log2Fold Change) | Q-PCR 16 °C vs 28 °C (log2Fold Change) |
|---|---|---|---|
Genes related to SFA synthesis | |||
 ACACA | Acetyl-CoA carboxylase | − 0.99 | − 1.14 ± 0.57 |
 FAS | Fatty acid synthase | − 1.52 | − 4.02 ± 0.53 |
 ELO | Long-chain fatty acid elongase | − 1.08 | − 2.43 ± 0.81 |
 VLCR | Very long-chain 3-oxoacyl-CoA reductase | − 1.07 | − 0.91 ± 0.50 |
 ME | Malic enzyme | − 1.03 | − 1.11 ± 0.62 |
Genes related to PUFA synthesis and the pentose phosphate pathway | |||
 PfaA | Polyketide synthase subunit A | − 0.88 | 0.35 ± 0.22 |
 PfaB | Polyketide synthase subunit B | − 0.79 | 0.05 ± 0.29 |
 PfaC | Polyketide synthase subunit C | − 0.66 | 0.12 ± 0.38 |
 PGD | Glucose-6-phosphate 1-dehydrogenase | 1.72 | 3.78 ± 0.57 |
Genes related to FA metabolism in organelles | |||
 ACADM-3 | Acyl-CoA dehydrogenase | − 1.39 | − 2.36 ± 0.65 |
 ACADM-5 | Acyl-CoA dehydrogenase | − 1.11 | − 1.80 ± 0.21 |
 ECHS-2 | Enoyl-CoA hydratase | 2.47 | 1.33 ± 0.22 |
 ECHS-6 | Enoyl-CoA hydratase | 1.54 | 1.42 ± 0.16 |
Genes related to glycolysis | |||
 HK | Glycolysis hexokinase | − 1.05 | − 0.62 ± 0.35 |
 PFKA | 6-Phosphofructokinase | − 1.48 | − 0.98 ± 0.21 |
 PGAM | Phosphoglycerate mutase | − 1.22 | 0.14 ± 0.42 |
 PC | Pyruvate carboxylase | − 1.79 | − 1.48 ± 0.031 |
Genes related to branched-chain amino acid degradation and the TCA cycle | |||
 TAT | Tyrosine aminotransferase | 1.77 | 2.5 ± 0.2 |
 HGD | Homogentisate 1,2-dioxygenase | 1.46 | 1.08 ± 0.19 |
 FAH | Fumarylacetoacetase | 1.17 | 2.23 ± 0.63 |
 OXCT | 3-Oxoacid CoA-transferase | 1.06 | 1.12 ± 0.11 |
 ACCAT | Acetyl-CoA C-acetyltransferase | 1.58 | 2.62 ± 0.39 |
 BCATC-1 | Ketol-acid reductoisomerase | − 1.12 | − 1.36 ± 0.41 |
 BCATC-2 | Ketol-acid reductoisomerase | 1.28 | 1.59 ± 0.27 |
 BCAT | Branched-chain amino acid aminotransferase | 1.26 | 2.69 ± 0.14 |
 BCKDH | 2-Oxoisovalerate dehydrogenase E1 component | 1.11 | 1.14 ± 0.07 |
 DBT | 2-Oxoisovalerate dehydrogenase E2 component | 1.52 | 3.46 ± 0.21 |
 GCDH | Glutaryl-CoA dehydrogenase | 1.07 | 0.52 ± 0.18 |
 LEU | 3-Isopropylmalate dehydratase | 2.49 | 3.08 ± 0.15 |
 IVD-2 | Isovaleryl-CoA dehydrogenase | 2.31 | 0.91 ± 0.28 |
 IVD-3 | Isovaleryl-CoA dehydrogenase | 1.66 | 2.64 ± 0.24 |
 MCCB | 3-Methylcrotonyl-CoA carboxylase beta subunit | 1.31 | 2.17 ± 0.27 |
 HMGCS | Hydroxymethylglutaryl-CoA synthase | − 1.19 | − 2.18 ± 0.31 |
 LSC | Succinyl-CoA synthetase | − 2.72 | − 1.3 ± 0.21 |