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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation

Fig. 5

Production of D-LA by L. bulgaricus ET45 in pulse fed-batch SSF. Experiment was conducted at 45 °C, pH 5.5 using 5 FPU/gLCB with 35% (w/v) total solid loading (Initial solid loading 5%, pulsed 5% pre-treated LCB (dil. acid pre-treated at 175 °C) and enzyme each at 36th, 48th, 60th, 72nd, 84th and 96th h). Diamond—glucose (g/L), triangle—D-LA (g/L), circle—cellobiose (g/L)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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