Fig. 3

Effects of gene disruption on cell growth, mannitol, and erythritol synthesis. a Molecular identification of 7 genes disruption (Ku70, YlAraDH1, YlAraDH2, YlMDH1, YlMDH2, YlXDH, YlEYD1 genes), no corresponding band can be amplified from the disruptants, but the band can be amplified for wild-type control strain (Y. lipolytica CGMCC7326, ery929), indicating Ku70, YlAraDH1, YlAraDH2, YlMDH1, YlMDH2, YlXDH, or YlEYD1 genes were disrupted individually. b Growth curves of different disruptants on liquid medium containing 300 g/L glucose. c Effects of various gene disruption on erythritol and mannitol biosynthesis, only mdh2 gene disruption can result in no mannitol synthesis. d, e The HPLC chromatography of wild-type strain and mdh2 gene disruption, showing no mannitol when the mdh2 gene was disrupted