Fig. 6

Results from duplicate fed-batch experiments on defined medium and pulses with acetate and aspartate (a, b) or acetate (c, d). E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss was used for cultivations. The batch media contained defined medium with 5 g l⁻¹ acetate and the onefold aspartate and vitamin concentration. A mixture of acetate and aspartate (a, b) or acetate alone (c, d) was added in pulses to obtain an acetate concentration of 4 g l⁻¹