Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W

Table 3 Diol production in medium containing different amounts of aspartate in E. coli W Δ4

Aspartate reduction	100% (8 mol%)	50% (4 mol%)	25% (2 mol%)	10% (0.8 mol%)
OD [−]	2.92 ± 0.08	0.84 ± 0.03	0.66 ± 0.02	0.62 ± 0.01
Total diols [g l⁻¹]	0.35 ± 0.01	0.12 ± 0.01	0.06 ± 0.01	0.03 ± 0.01

E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss was used for cultivation. Media contained 5 g l⁻¹ acetate and the indicated percentage of 0.88 g l⁻¹ aspartate. The ratio of aspartate to acetate is given in % of moles. Cultures were inoculated at OD₆₀₀ = 0.5. Mean values and standard deviations were calculated from biological triplicates

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