Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W

Table 5 Process performance parameters of E. coli W Δ4 in batches with aspartate and acetate

Parameter	Aspartate phase	Acetate phase	Total
Y_diols/Ace [Cmol Cmol⁻¹]	0.18 ± 0.09	0.13 ± 0.03	0.14 ± 0.01
Y_diols/Ace [g g⁻¹]	0.14 ± 0.07	0.09 ± 0.02	0.10 ± 0.01
Y_diols/S [Cmol Cmol⁻¹]	0.11 ± 0.05	0.13 ± 0.03	0.12 ± 0.01
Y_X/S [Cmol Cmol⁻¹]	0.36 ± 0.09	0.29 ± 0.05	0.31 ± 0.01
Y_CO2/S [Cmol Cmol⁻¹]	0.71 ± 0.18	0.84 ± 0.11	0.78 ± 0.01
Carbon balance [%]	105 ± 9	112 ± 10	117 ± 1

E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss was used for cultivation. Yields are either calculated per acetate consumed (Y_diols/Ace) or per acetate and aspartate consumed (Y_diols/S, Y_X/S, Y_CO2/S, X = biomass, Ace = acetate, S = substrate (acetate + aspartate)). The medium contained the onefold aspartate (0.88 g l⁻¹) and vitamin concentration. Mean values and standard deviations were calculated from biological duplicates. The theoretical yield is 0.5 Cmol diols per Cmol acetate

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