Fig. 6

Comparison of different domains of SmBdh with other known Bdh enzymes. a Comparison of α6 helix of the SmBdh and KpBdh. SmBdh is shown as grey cartoon covered with semi-transparent surface. NAD ⁺ and acetoin molecules are shown in sticks representation. KpBdh is shown as cyan cartoon. The larger α6 helix of the KpBdh is covered in cyan mesh to illustrate the additional volume obstructing the catalytic cleft. b Sequence alignment of SmBdh (accession #AFH00999), EcBdh (accession #AFM58915), KpBdh (PDB 1GEG), LlBdh (UniProtKB—D2BQ24), GoBdh (PDB 3WTC), BxBdh (PDB 5JY1), CgBdh (PDB 3A28), BcBdh (PDB 4WEO) using ClustalW program. The 11 amino acid deletion region in SmBDH is shown with a black box. Red lines, alpha helix; blue lines, beta strand; green lines, alpha turn; black line, C-terminal extension in SmBdh