Table 2 Biomass, glucose uptake and production of ethanol, glycerol and acetate of the constructed S. cerevisiae strains under anaerobic conditions

µ

[h⁻¹]

0.316 ± 0.005

0.201 ± 0.006

(p < 0.0001)

0.202 ± 0.003

(p < 0.0001)

0.235 ± 0.003

(p < 0.0001)

Y_BM/Glc

[gCDW/g]

0.0971 ± 0.0007

0.0845 ± 0.0009

(p < 0.0001)

0.0774 ± 0.0003

(p < 0.0001)

0.0741 ± 0.0010

(p < 0.0001)

r_Glc

[mmol/gCDW/h]

17.26 ± 0.27

12.66 ± 0.66

(p = 0.0004)

14.18 ± 0.60

(p = 0.0012)

17.44 ± 0.63

(p = 0.6712)

q_Glc

[mmol/L/h]

3.35 ± 0.02

1.74 ± 0.07

(p < 0.0001)

2.60 ± 0.11

(p = 0.0003)

3.04 ± 0.03

(p < 0.0001)

r_Eth

[mmol/gCDW/h]

26.09 ± 0.18

20.07 ± 0.84

(p = 0.0003)

24.82 ± 0.10

(p = 0.0004)

28.77 ± 0.76

(p = 0.0040)

q_Eth

[mmol/L/h]

5.06 ± 0.05

2.76 ± 0.05

(p < 0.0001)

4.55 ± 0.10

(p = 0.0018)

5.02 ± 0.03

(p = 0.3346)

Y_Eth/Glc

[mol/mol]

1.58 ± 0.01

1.62 ± 0.01

(p = 0.0042)

1.73 ± 0.03

(p = 0.0007)

1.74 ± 0.03

(p = 0.0014)

r_Gly

[mmol/gCDW/h]

3.90 ± 0.22

2.89 ± 0.24

(p = 0.0059)

2.49 ± 0.16

(p = 0.0009)

3.39 ± 0.04

(p = 0.0172)

Y_Gly/Glc

[mol/mol]

0.220 ± 0.003

0.201 ± 0.004

(p = 0.0040)

0.162 ± 0.006

(p = 0.0001)

0.185 ± 0.003

(p < 0.0001)

r_Ac

[mmol/gCDW/h]

n.d.

n.d.

n.d.

n.d.

Y_Ac/Glc

[mol/mol]

n.d.

n.d.

n.d.

n.d.

r_ATP-NGAM

[mmol/gCDW/h]

0.30

3.66

8.33

9.59