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Table 2 Biomass, glucose uptake and production of ethanol, glycerol and acetate of the constructed S. cerevisiae strains under anaerobic conditions

From: ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production

  5D (E.V.) 5D-atp2 5D-atpAGD 5D-atpAGD (2A)
µ
[h−1]
0.316 ± 0.005 0.201 ± 0.006
(p < 0.0001)
0.202 ± 0.003
(p < 0.0001)
0.235 ± 0.003
(p < 0.0001)
YBM/Glc
[gCDW/g]
0.0971 ± 0.0007 0.0845 ± 0.0009
(p < 0.0001)
0.0774 ± 0.0003
(p < 0.0001)
0.0741 ± 0.0010
(p < 0.0001)
rGlc
[mmol/gCDW/h]
17.26 ± 0.27 12.66 ± 0.66
(p = 0.0004)
14.18 ± 0.60
(p = 0.0012)
17.44 ± 0.63
(p = 0.6712)
qGlc
[mmol/L/h]
3.35 ± 0.02 1.74 ± 0.07
(p < 0.0001)
2.60 ± 0.11
(p = 0.0003)
3.04 ± 0.03
(p < 0.0001)
rEth
[mmol/gCDW/h]
26.09 ± 0.18 20.07 ± 0.84
(p = 0.0003)
24.82 ± 0.10
(p = 0.0004)
28.77 ± 0.76
(p = 0.0040)
qEth
[mmol/L/h]
5.06 ± 0.05 2.76 ± 0.05
(p < 0.0001)
4.55 ± 0.10
(p = 0.0018)
5.02 ± 0.03
(p = 0.3346)
YEth/Glc
[mol/mol]
1.58 ± 0.01 1.62 ± 0.01
(p = 0.0042)
1.73 ± 0.03
(p = 0.0007)
1.74 ± 0.03
(p = 0.0014)
rGly
[mmol/gCDW/h]
3.90 ± 0.22 2.89 ± 0.24
(p = 0.0059)
2.49 ± 0.16
(p = 0.0009)
3.39 ± 0.04
(p = 0.0172)
YGly/Glc
[mol/mol]
0.220 ± 0.003 0.201 ± 0.004
(p = 0.0040)
0.162 ± 0.006
(p = 0.0001)
0.185 ± 0.003
(p < 0.0001)
rAc
[mmol/gCDW/h]
n.d. n.d. n.d. n.d.
YAc/Glc
[mol/mol]
n.d. n.d. n.d. n.d.
rATP-NGAM
[mmol/gCDW/h]
0.30 3.66 8.33 9.59
  1. Specific and volumetric rates are reported for the period where all strains showed exponential growth (2.5–7 h; see Fig. 2). The values of the yields are reported for the entire cultivation period (0–10 h). The data are from triplicate experiments and the error ranges represent standard deviations. p values for a two-sample t test are given with respect to the control strain 5D (E.V.) indicating whether a significant change has occurred
  2. YBM/Glc, biomass yield; rGlc, specific glucose uptake rate; qGlc, volumetric glucose uptake rate; rEth, specific ethanol production rate; qEth, volumetric ethanol productivity; YEth/Glc, ethanol yield; rGly, specific glycerol production rate; YGly/Glc, glycerol yield; rAc, specific acetate production rate; YAc/Glc, acetate yield; rATP-NGAM, non-growth-associated ATP maintenance demand (estimated from flux balance calculations)
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