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Table 3 Growth rate, glucose uptake and production of ethanol, glycerol and acetate of the constructed S. cerevisiae strains under growth-decoupled (nitrogen-starved) cultivation

From: ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production

  5D (E.V.) 5D-atp2 5D-atpAGD 5D-atpAGD (2A)
µ
[h−1]
 ~ 0  ~ 0  ~ 0  ~ 0
rGlc
[mmol/gCDW/h]
2.07 ± 0.05 1.65 ± 0.05
(p = 0.0004)
2.85 ± 0.06
(p < 0.0001)
3.24 ± 0.16
(p = 0.0003)
qGlc
[mmol/L/h]
1.73 ± 0.04 1.44 ± 0.02
(p = 0.0004)
2.32 ± 0.10
(p = 0.0007)
3.07 ± 0.08
(p < 0.0001)
rEth
[mmol/gCDW/h]
3.07 ± 0.03 2.50 ± 0.03
(p < 0.0001)
4.53 ± 0.11
(p < 0.0001)
5.71 ± 0.55
(p = 0.0011)
qEth
[mmol/L/h]
2.56 ± 0.07 2.19 ± 0.07
(p = 0.0033)
3.68 ± 0.17
(p = 0.0005)
5.40 ± 0.38
(p = 0.0002)
YEth/Glc
[mol/mol]
1.54 ± 0.00 1.71 ± 0.04
(p = 0.0017)
1.62 ± 0.04
(p = 0.0294)
1.75 ± 0.04
(p = 0.0011)
rGly
[mmol/gCDW/h]
0.386 ± 0.017 0.331 ± 0.005
(p = 0.0058)
0.444 ± 0.009
(p = 0.0060)
0.490 ± 0.011
(p = 0.0008)
YGly/Glc
[mol/mol]
0.172 ± 0.003 0.192 ± 0.005
(p = 0.0039)
0.138 ± 0.005
(p = 0.0006)
0.144 ± 0.002
(p = 0.0002)
rAc
[mmol/gCDW/h]
0.0503 ± 0.0041 0.0413 ± 0.0108
(p = 0.2506)
0.0666 ± 0.0109
(p = 0.0713)
0.0779 ± 0.0058
(p = 0.0025)
YAc/Glc
[mol/mol]
0.0198 ± 0.0014 0.0228 ± 0.0041
(p = 0.2931)
0.0230 ± 0.0038
(p = 0.2309)
0.0249 ± 0.0028
(p = 0.0443)
rATP-NGAM
[mmol/gCDW/h]
2.88 2.33 4.31 5.47
  1. Specific and volumetric rate values are reported for the first 5.5 h of cultivation. Yields are reported for the entire period of cultivation (0–10 h). The data are from triplicate experiments and the error ranges represent standard deviations. p values for a two-sample t test are given with respect to the control strain 5D (E.V.) indicating whether a significant change has occurred
  2. YBM/Glc, biomass yield; rGlc, specific glucose uptake rate; qGlc, volumetric glucose uptake rate; rEth, specific ethanol production rate; qEth, volumetric ethanol productivity; YEth/Glc, ethanol yield; rGly, specific glycerol production rate; YGly/Glc, glycerol yield; rAc, specific acetate production rateYAc/Glc, acetate yield; rATP-NGAM, non-growth-associated ATP maintenance demand (estimated from flux balance calculations)