Skip to main content

Table 3 Primers used in this study

From: Rapid optimisation of cellulolytic enzymes ratios in Saccharomyces cerevisiae using in vitro SCRaMbLE

Primer name Primer sequence
loxP pair SynXIV F 5′-CCACTAGTCTTCTAGAAAGCATTTTCCCATGAACT-3′
loxP pair SynXIV F 5′-CAAAGAGGTTTAGACGGCCAGGCGTATTCTGATGA-3′
qPCR CEL5A F 5′-AGTGGTGTAAGGTTCGCTGG-3′
qPCR CEL5A R 5′-GCTGCATTTGACCGATACCG-3′
qPCR hphMX4 F 5′-CTTCGATGTAGGAGGGCGTG-3′
qPCR hphMX4 R 5′-TCAGGCTCTCGCTGAATTCC-3′
qPCR CEL3A F 5′-GTAGGCTCCAAGACGTCTGG-3′
qPCR CEL3A R 5′-CGAATCGAACACCCAATGGC-3′
qPCR TAF10 F 5′-ATATTCCAGGATCAGGTCTTCCGTAGC-3′
qPCR TAF10 R 5′-GTAGTCTTCTCATTCTGTTGATGTTGTTGT-3′