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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant

Fig. 1

Phenotypic investigation of P. oxalicum strains ∆PoxKu70 and OXPoxGA15A, grown on a two-layer agar gel plate (a) and their RSDE activity in liquid medium (b). The two-layer agar gel plate consisted of an upper layer containing ball-milled Avicel (0.5% w/v) and natural raw cassava flour (RNCF, 1% w/v), and a lower layer of MMM without carbon source. Carbon sources used in liquid medium were the same as that in the top layer of the two-layer plate. RSDE activity was determined using RNCF as the substrate. ** (p ≤ 0.01) indicates significant difference between the OXPoxGA15A and ∆PoxKu70 by Student’s t test. RSDE raw starch-degrading enzyme, MMM modified minimal medium

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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