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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant

Fig. 3

Effects of pH and temperature on RSDE activity of P. oxalicum strains A2-13 and OXPoxGA15A. a pH profile of RSDE activity. Enzyme activity was measured at pHs between 3.0 and 7.0 in 0.1 M citrate–phosphate buffer at 37 °C. b Temperature profile of RSDE activity. c Effect of pH on RSDE stability. d Thermal stability of RSDE activity. P. oxalicum strains were cultured for 6 days under the optimal culture conditions. In a and b, the highest RSDE activity of A2-13 and OXPoxGA15A was set as 100%. In c and d, the RSDE activity of untreated A2-13 and OXPoxGA15A was set as 100%. Each experiment was independently performed three times. Each data point represents mean ± SD

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