Table 2 Fermentation parameters for the strains harboring the improved IrrE at different cultivation temperature
From: Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast
FAP | Strains | µ (h−1) | rglu (g/L/h) | Ethanol titer (g/L) | reth(g/L/h) | Ethanol yield (%) |
|---|---|---|---|---|---|---|
38 ℃ | BY4742/pRS416 | 0.44 ± 0.03 | 1.32 | 8.52 ± 0.18 | 0.57 ± 0.012 | 85.0 |
BY4742/IrrE | 0.56 ± 0.03 | 1.50 | 8.73 ± 0.13 | 0.58 ± 0.008 | 87.1 | |
BY4742/I12 | 0.78 ± 0.01 | 1.64 | 8.84 ± 0.06 | 0.74 ± 0.005 | 88.3 | |
BY4742/I24 | 0.73 ± 0.01 | 1.65 | 8.90 ± 0.08 | 0.74 ± 0.007 | 88.9 | |
BY4742/I37 | 0.76 ± 0.01 | 1.66 | 8.76 ± 0.16 | 0.73 ± 0.013 | 87.5 | |
42 ℃ | BY4742/pRS416 | 0.03 ± 0.01 | 0.21 | 1.33 ± 0.24 | 0.038 ± 0.007 | 13.3 |
BY4742/IrrE | 0.162 ± 0.01 | 0.89 | 8.34 ± 0.23 | 0.14 ± 0.004 | 83.3 | |
BY4742/I12 | 0.163 ± 0.02 | 0.92 | 8.90 ± 0.13 | 0.15 ± 0.002 | 88.9 | |
BY4742/I24 | 0.106 ± 0.03 | 0.32 | 6.26 ± 0.76 | 0.11 ± 0.006 | 62.5 | |
BY4742/I37 | 0.119 ± 0.01 | 0.34 | 7.05 ± 0.78 | 0.12 ± 0.007 | 70.4 |