Fig. 3

Thermal unfolding of mgLPMO10. The apparent melting temperatures (T_m(app)) were determined by differential scanning calorimetry (DSC) by heating the full-length mgLPMO10-holo (approximately 0.4 g/L) at 1 °C/min from 25 °C to 100 °C in 50 mM sodium phosphate buffer (pH 6.0). The experiment was repeated for the apo version of the protein (mgLPMO10^apo) and the holo catalytic domain (mgLPMO10^CD)