Fig. 2

Activity of TausLPMO9B on PASC. a HPAEC–PAD chromatogram for products released after 24 h incubation at 45 °C, pH 5.0, in the presence (continuous line) or absence (dotted line) of 1 mM ascorbic acid (TausLPMO9B chromatograms are shown in blue, NcLPMO9C in green, NcLPMO9F in red, and the negative control in black). The reactions contained 0.1% (w/v) PASC and1 µM enzyme. b Accumulation of cellobionic acid over time in a reaction with 1 µM TausLPMO9B, 0.1% (w/v) PASC and 1 mM ascorbic acid. Cellobionic acid release by TausLPMO9B was quantified by intrapolation of the integrated peak area of the C1-oxidized dimer with a standard curve of cellobionic acid standard solutions (10–50 µM). (Note that cellobionic acid corresponds to only a fraction of the LPMO products as longer C1-oxidized cello-oligosaccharides were not converted to dimers before HPAEC–PAD analysis)