Table 6 Details of enzymatic dilutions, solution concentrations and total solution size, used in the enzymatic saccharification
Component | Final concentration | Enzymatic master mix for 36 samples | Total in 1000 µL reaction |
|---|---|---|---|
Water for hydrothermal pretreatment | – | – | 340 µL |
Biomass | 10 mg/ml (solution) | – | 10 mg |
50 mM citrate buffer (pH 4.8) | 50 mM | 29.23 mL | 649.468 mL |
CTec enzyme | 9 enzymatic units per gram of AIR | 21.546 µL | 0.0532 µL |
HTec enzyme | 1 enzymatic unit per gram of AIR | 2.394 µL | 0.4788 µL |