Table 3 Enzymes involved in lipid metabolism in Saccharomyces cerevisiae model, iNL800 [70], Yarrow lipolytica model, iNL895 [37] and Cutaneotrichosporon oleaginosus ATCC 20509 model, iNP636_Coleaginosus_ATCC20509 (this study)

EC 6.2.1.1

Acetyl-coenzyme A synthetase 1

Y

–

–

EC 6.2.1.1

Acetyl-coenzyme A synthetase 2

Y

Y

Y

EC 1.3.1.9

Fatty acid synthase subunit beta

Y

Y (2)

Y

EC 2.3.1.86

Fatty acid synthase subunit alpha

Y

Y (2)

Y

EC 2.7.7.41

Phosphatidate cytidylyltransferase

Y

Y (2)

Y

EC 2.7.8.11

CDP-diacylglycerol–inositol 3-phosphatidyltransferase

Y

Y

Y

EC 2.7.8.8

CDP-diacylglycerol–serine O-phosphatidyltransferase

Y

Y

Y

EC 2.7.1.30

Glycerol kinase

Y

Y

Y (2)

EC 1.1.1.8

Glycerol-3-phosphate dehydrogenase (NAD( +))

Y (2)

Y

Y (2)

EC 2.3.1.51

Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase

Y

Y (2)

Y

EC 2.3.1.20

Diacylglycerol O-acyltransferase

Y

Y

Y

EC 2.3.1.158

Phospholipid:diacylglycerol acyltransferase

Y

Y

Y

EC 3.1.1.3

Triacylglycerol lipase

Y (3)

Y (2)

Y (2)

EC 2.3.1.26

Acyl-CoA:sterol acyltransferase

Y

Y

Y

EC 1.14.19.1

Acyl-CoA desaturase

Y

Y

Y

EC 1.14.19.6

∆12 Fatty acid desaturase

–

Y

Y

EC 1.3.3.6

Acyl-coenzyme A oxidase

Y

Y (3)

Y

EC 2.3.1.16

3-ketoacyl-CoA thiolase, peroxisomal

Y

Y

Y (2)

EC 2.3.3.8

ATP-citrate lyase, subunit a

–

Y

Y

EC 2.3.3.8

ATP-citrate lyase, subunit b

–

Y

Y

EC 1.1.1.38

NAD-dependent malic enzyme, mitochondrial

Y

Y

Y

EC 6.4.1.2

Acetyl-CoA carboxylase

Y

Y

Y