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Table 1 Primers used in this study

From: Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels

Primer name

Sequence 5′→3′

G_Ptet_for

ACAGCTATGACCGCGGCCGCttaagacccactttcacatttaag

G_Ptet_rev

TTCGTAATCATGGTCATatgagttaacctcctgtcgac

G_pMTL8312_for

catatgaccatgattacgaattc

G_pMTL8312_rev

gcggccgcggtcatagct

G_SrnfH_for

TCGTTAGTCGACAGGAGGTTAACTCATatgtggagccacccgcagttc

G_SrnfH_rev

GCTAGCGCCATTCGCCATTCAGGCTGCGCActaatggtgatggtgatggtgtttgg

G_pMTL_tet_for

tgcgcagcctgaatggcg

G_pMTL_tet_rev

catatgagttaacctcctg

RnfG_His_for

catcaccatcaccattaggagggaaaagtaaagatg

RnfG_His_rev

gtgggcgcctttcgacaaattctggtaaac

  1. Underlined letters specify restriction sites, capital letters highlight the homologous extensions for Gibson assembly, and bold letters are extension sited for addition of an His-tag via site-directed mutagenesis
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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