Fig. 1

Simplified diagram of the Shikimate and Ehrlich pathways in K. marxianus for 2-phenylethanol biosynthesis. Arrows with dashed lines represent more than one reaction. G-6-P: glucose 6-phosphate; PEP: phosphoenolpyruvate; E-4-P: erythrose-4-phosphate; DAHP: 3-deoxy-d-arabinoheptulosonate-7-phosphate; PABA: para-aminobenzoate; 2-PE: 2-phenylethanol; 2-PEAc: 2-phenylethyl acetate. ARO3/ARO4: DAHP synthase; ABZ1: PABA synthase; ARO7: chorismate mutase; PHA2: prephenate dehydratase; ARO8/ARO9: aromatic aminotransferase; ARO10: phenylpyruvate decarboxylase; ADH: alcohol dehydrogenase; PAR phenylacetaldehyde reductase; EAT1: ethanol acetyltransferase