Fig. 3
Shikimate pathway enzyme screening. ARO4 and ARO7 variants were overexpressed from plasmids with S. cerevisiae TDH3 promoter (PScTDH3) and were screened for 2-phenylethyl acetate (2-PEAc) biosynthesis. 2-PEAc was quantified by GC-FID after 18 h cultivation at 30 °C from an initial OD600 of 0.05 in 25 mL SD-H medium. The first control strain (cntrl.1) was K. marxianus CBS 6556 ura3Δ his3Δ harboring a low-copy number empty vector. The second control strain (cntrl.2) is K. marxianus CBS 6556 ura3Δ his3Δ abz1::(PScTDH3)KmARO4K221L- (PScTDH3)KmARO10 harboring the empty vector. All experiments were performed in biological triplicates. Bars represent the mean, while error bars represent the standard deviation