Fig. 1

Maximum-likelihood phylogenetic tree of all currently characterized AA10 LPMO catalytic modules. Full-length protein sequences were retrieved from GenBank via the CAZy database and manually truncated to remove the signal peptide and C-terminal domains (guided in part by SignalP and BLASTP analysis). The catalytic sequences were then aligned using MUSCLE and manually refined to preserve the position of the catalytic residues prior to maximum-likelihood analysis using RAxML 8.2.10 utilizing a JTT matrix-based nucleotide substitution model and automatic halting bootstraps (702 bootstraps). Three characterized AA9 LPMO catalytic module sequences with different regioselective activities on chitin were used as outgroups (GtLPMO9B [131], AN1602 [132], and NcLPMO9D [133]). The input and output files are provided as Additional file1: S1 and Additional file2: S2