Fig. 2

Engineering production of 10-methylene BFAs in Y. lipolytica. To measure fatty acid species, plate lipid accumulation assays were carried out and individual fatty acids species were quantified by GC-FID and reported as the percentage of total C16 and C18 fatty acid species. a Strain NS1009 was transformed with replicating plasmids containing the indicated bfaB genes from M. smegmatis (pNC915), M. vanbaaleni (pNC917), H. subflava (pNC918), C. glyciniphilum (pNC919), R. opacus (pNC920), A. subbeticus (pNC923), K. aerolata (pNC924), M. gilvum (pNC925), M. indicus (pNS926), T. fusca (pNC921), T curvata (pNC922). C10-methylene fatty acids were measured. * Indicates NS1117. b Strain NS1009 was transformed with integrating constructs expressing tmpB genes from D. postgatei (pNC996), D. balticum (pNC998), D. toluoica (pNC1000), M. hydrocarbonclasticus (pNC1002), T. denitrificans (pNC1004), T. halophila (pNC1006). The sum of all C8 and C10-methylene fatty acids was measured. c Fatty acid profiles were measured for NS1009 and strains containing one (NS1195) and two (NS1647) integrated copies of the T. curvata bfaB gene. The sum of all saturated, monounsaturated, and C8 and C10-methylene fatty acids was measured, and the mean and range of samples from duplicate biological experiments is shown