Fig. 3

Engineering production of 10-methyl BFAs in Y. lipolytica. To measure fatty acid species, plate lipid accumulation assays were carried out and individual fatty acids species were quantified by GC-FID and reported as the percentage of total C16 and C18 fatty acid species. a NS1117 expressing the T. curvata bfaB gene was transformed with CEN plasmids expressing bfaA genes from C. glutamicum (pNC1024), T. fusca (pNC1025) and T. curvata (pNC984) and a no plasmid control. C8- and C10-methylene (blue) and methyl (red) fatty acids were measured. Signal in the no plasmid control represents GC background signal in this experiment. b NS1009 was transformed with integrating constructs expressing T. curvata bfaA-B and bfaB-A fusion proteins. C8- and C10-methylene (blue) and methyl (red) fatty acids were measured. c Saturated, monounsaturated, 8- and 10-methylene, and 8- and 10-methyl fatty acid species were measured for NS1009 and strains expressing one (NS1190) or two (NS1227) integrated copies of T. curvata bfaA-B. The mean and range of samples from duplicate biological experiments is shown