Production of 10-methyl branched fatty acids in yeast

Table 1 BFA production in bfaAB and tmpBA expressing E. coli

E. coli vector	Donor organism	% oleic acid conversion to 10-methylstearic acid
E. coli vector	bfaAB operon	% oleic acid conversion to 10-methylstearic acid
pNC704	Mycobacterium smegmatis	4.9 ± 0.6%
pNC721	Mycobacterium vanbaaleni	0
pNC755	Amycolicicoccus subflavus	0
pNC757	Corynebacterium glyciniphilum	0
pNC904	Rhodococcus opacus	1.2 ± 0.2%
pNC905	Thermobifida fusca	22.0 ± 0.3%
pNC906	Thermomonospora curvata	38.3 ± 0.5%
pNC907	Corynebacterium glutamicum	0
pNC908	Agromyces subbeticus	0
pNC910	Mycobacterium gilvum	0
pNC911	Mycobacterium sp. indicus	0
pNC53	Empty control vector	0

	tmpBA operon	% palmitoleic acid conversion to 10-methylenepalmitic acid
pNC1071	Desulfobacter postgatei	31.5 ± 0.1%
pNC1072	Desulfobacter balticum	0
pNC1073	Desulfobacula toluolica	11.8 ± 0.3%
pNC1074	Marinobacter hydrocarbonoclasticus	45.3 ± 0.3%
pNC1076	Thiohalospira halophila	70.4 ± 0.3%
pNC53	Empty control vector	0

E. coli Top10 cells containing the indicated plasmid were cultivated in LB medium supplemented with 100 mg/L oleic acid (for bfaAB operons) or 100 mg/L palmitoleic acid (for tmpBA operons) and antibiotic. Percent conversion was measured by dividing the 10-methyl/10-methylene fatty acid content by the sum of the remaining precursor fatty acid and the 10-methyl/10-methylene fatty acids present in E. coli cell mass

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