Fig. 4

Effect of different carbohydrates on C. thermocellum cellulase activity. 0.5 g/L initial Avicel was incubated with 10 mg/g C. thermocellum cellulase and activity was estimated by measuring the decrease in OD₆₀₀ after 24 h. The cellulase enzyme was prepared by concentrating the supernatant from a C. thermocellum fermentation on 20 g/L Avicel. The error bars show the SD for n = 2. XOS xylooligosaccharides, AX arabinoxylan, control broth—see “Materials and methods”. Glucomannan, galactomannan, and xyloglucan used here are all oligosaccharide mixtures, prepared by partial enzymatic hydrolysis of the respective carbohydrate