Fig. 7
From: Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose
The majority (> 80%) of intact Cel7A (WTintact- or E212Qintact) exhibited short binding lifetimes on cellulose fibrils, with < 20% appearing to be effectively ‘immobilized’. A large fraction (~ 75%) of the Cel7A catalytic domains (WTcore and E212Qcore) also exhibited short binding lifetimes. Approximately 20% of the core enzyme bound for ~ 100 s, with only 6–7% ‘immobilized’