Fig. 5

SDS-PAGE/Western immunoblot of the Synechocystis strains created in this study (Table 2) analysed for the presence of phosphoenolpyruvate synthase (PPSA) and ethylene-forming enzyme (EFE). A SDS-PAGE loaded with 3 µg of protein crude extract from the Synechocystis strains created in this study under standard treatment (Table 2) and B Western immunoblot using anti-Flag antibody for the Synechocystis engineered strains (Table 2) under standard treatment. Standard treatment is BG11+ 50 mM Tris pH 8.0, 50 mM NaHCO₃, Km (25 µg mL⁻¹), Cm (20 µg mL⁻¹) and 5 µM of NiCl₂ and 20 µE m⁻² s⁻¹. The upper band corresponds to PPSA (approximate molecular weight 91.17 and 92.56 kDa for PPSA₆₈₀₃ and PPSA₇₀₀₂, respectively) and the lower band to EFE (approximate molecular weight 42.16 kDa)