Fig. 7

SDS-PAGE/Western immunoblot of the Synechocystis strains created in this study (Table 2) analysed for the presence of phosphoenolpyruvate carboxylase (PEPc). A SDS-PAGE loaded with 31 µg of protein crude extract from Synechocystis strains created in this study under standard treatment (Table 2) and B Western immunoblot using anti-PEPc for the Synechocystis engineered strains (Table 2) in standard treatment. Standard treatment is BG11+ 50 mM Tris pH 8.0, 50 mM NaHCO₃, Km (25 µg mL⁻¹), Cm (20 µg mL⁻¹), 5 µM of NiCl₂ and 20 µE m⁻² s⁻¹. PEPc₆₈₀₃ and PEPc₇₀₀₂ corresponds to purified protein (100 ng loaded) of PEPc from Synechocystis PCC 6803 and Synechococcus PCC 7002, respectively