Table 1 Plasmids used in the study

 pEZ15Asp (pEZ)

Shuttle vector contains Z. mobilis origin and E. coli origin p15A; Sp^R;Biobrick-compatible

Lab stock

 pEZ-KT

Sp^R; pEZ containing: kdcA gene from L. lactic driven by inducible promoter Ptet

This work

 pEZ-A0

Sp^R; pEZ containing; ilvC and ilvD genes from Z. mobilis driven by inducible promoter Ptet

This work

 pEZ-A1

Sp^R; pEZ containing: als, ilvC and ilvD genes from Z. mobilis driven by inducible promoter Ptet

This work

 pEZ-A2

Sp^R; pEZ containing: codon-optimized EcAls gene from E. cloacae, ilvC and ilvD genes from Z. mobilis driven by Ptet

This work

 pEZ-A3

Sp^R; pEZ containing: codon-optimized BlAls from B. licheniformis, ilvC and ilvD from Z. mobilis driven by Ptet

This work

 pEZ-A4

Sp^R; pEZ containing: codon-optimized BsAls gene from B. subtilis, ilvC and ilvD genes from Z. mobilis driven by Ptet

This work

 pEZ-B

Sp^R; pEZ containing: codon-optimized BsAls gene from B. subtilis driven by inducible promoter Ptet

Plasmids for recombination

 pUC57

Vector contains E. coli origin; Amp^R

 pUC-KT1

Amp^R, Cm^R; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Ptet and the upstream and downstream of ZMO0038

This work

 pUC-KT2

Amp^R, Cm^R; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Ptet and the upstream and downstream of pZYM36-005

This work

 pUC-KG1

Amp^R, Cm^R; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Pgap and the upstream and downstream of ZMO0038

This work

 pUC-KG2

Amp^R, Cm^R; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Pgap and the upstream and downstream of pZYM36-005

This work

 pUC-KE

Amp^R, Cm^R; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Peno and the upstream and downstream of ZMO0038

This work