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Table 1 Plasmids used in the study

From: Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production

Plasmids Description Source
 pEZ15Asp (pEZ) Shuttle vector contains Z. mobilis origin and E. coli origin p15A; SpR;Biobrick-compatible Lab stock
 pEZ-KT SpR; pEZ containing: kdcA gene from L. lactic driven by inducible promoter Ptet This work
 pEZ-A0 SpR; pEZ containing; ilvC and ilvD genes from Z. mobilis driven by inducible promoter Ptet This work
 pEZ-A1 SpR; pEZ containing: als, ilvC and ilvD genes from Z. mobilis driven by inducible promoter Ptet This work
 pEZ-A2 SpR; pEZ containing: codon-optimized EcAls gene from E. cloacae, ilvC and ilvD genes from Z. mobilis driven by Ptet This work
 pEZ-A3 SpR; pEZ containing: codon-optimized BlAls from B. licheniformis, ilvC and ilvD from Z. mobilis driven by Ptet This work
 pEZ-A4 SpR; pEZ containing: codon-optimized BsAls gene from B. subtilis, ilvC and ilvD genes from Z. mobilis driven by Ptet This work
 pEZ-B SpR; pEZ containing: codon-optimized BsAls gene from B. subtilis driven by inducible promoter Ptet  
Plasmids for recombination  
 pUC57 Vector contains E. coli origin; AmpR  
 pUC-KT1 AmpR, CmR; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Ptet and the upstream and downstream of ZMO0038 This work
 pUC-KT2 AmpR, CmR; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Ptet and the upstream and downstream of pZYM36-005 This work
 pUC-KG1 AmpR, CmR; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Pgap and the upstream and downstream of ZMO0038 This work
 pUC-KG2 AmpR, CmR; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Pgap and the upstream and downstream of pZYM36-005 This work
 pUC-KE AmpR, CmR; pUC containing: chloramphenicol resistant gene, kdcA gene from L. lactic driven by Peno and the upstream and downstream of ZMO0038 This work