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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Improved bioethanol productivity through gas flow rate-driven self-cycling fermentation

Fig. 3

Ergosterol and Tween 80 supplementation. SCF was performed using medium without (cycles 1–4, 9–14, and 19–24) or with (cycles 5–8, 15–18, and 25–28) the supplementation of ergosterol (0.02 g/L) and Tween 80 (0.8 g/L). The cycle time (a), pH of the culture (b), as well as gas flow rate (c) and its slope (d) were reported. The cycle numbers were labeled at the top of each figure, with the exception of a where the cycle numbers were indicated on the x-axis. Cycles with supplementation were shaded in bd. Underlined cycle numbers indicate cycles that did not meet criteria for automated cycling and that were thus manually triggered. Cycle 15 and part of cycle 16 were removed from c and d due to excessive flow of nitrogen entering the fermenter as a result of nitrogen regulator failure

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