Table 1 Plasmids and strains used in this study
From: Protein-based biorefining driven by nitrogen-responsive transcriptional machinery
Plasmids | Description | Origin | Resistance | References |
|---|---|---|---|---|
pSA69 | PLlacO1: alsS-ilvC-ilvD | p15A | Kanamycin | [17] |
pYX97 | PLlacO1: leuDH-kivD-yqhD; lacI | colE1 | Ampicillin | [3] |
pLM1 | PLlacO1: alsS-ilvC-ilvD-avtA | p15A | Kanamycin | This study |
pLM2 | rrnBp1: alsS-ilvC-ilvD-avtA | p15A | Kanamycin | This study |
pLM3 | rrnBp1: leuDH-kivD-yqhD; lacI | colE1 | Ampicillin | This study |
pLM4 | J23100: alsS-ilvC-ilvD-avtA | p15A | Kanamycin | This study |
pLM5 | J23100: leuDH-kivD-yqhD; lacI | colE1 | Ampicillin | This study |
pLM6 | glnAp2: alsS-ilvC-ilvD-avtA | p15A | Kanamycin | This study |
pLM7 | glnAp2: leuDH-kivD-yqhD; lacI | colE1 | Ampicillin | This study |
pLM8 | argTp: alsS-ilvC-ilvD-avtA | p15A | Kanamycin | This study |
pLM9 | argTp: leuDH-kivD-yqhD; lacI | colE1 | Ampicillin | This study |
pLMg1 | argTp: gfp | p15A | Kanamycin | This study |
pLMg2 | astCp: gfp | p15A | Kanamycin | This study |
pLMg3 | ddpXp: gfp | p15A | Kanamycin | This study |
pLMg4 | glnHp2: gfp | p15A | Kanamycin | This study |
pLMg5 | glnKp: gfp | p15A | Kanamycin | This study |
pLMg6 | nacp: gfp | p15A | Kanamycin | This study |
pLMg7 | pabB: gfp | p15A | Kanamycin | This study |
pLMg8 | patAp: gfp | p15A | Kanamycin | This study |
pLMg9 | puuPp: gfp | p15A | Kanamycin | This study |
pLMg10 | rutAp: gfp | p15A | Kanamycin | This study |
pLMg11 | yhdWp: gfp | p15A | Kanamycin | This study |
Strain | Description | References |
|---|---|---|
XL10-Gold | TetrΔ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F´ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] | Agilent technologies |
JCL16 | BW25113/F’ [traD36, proAB+, lacIqZΔM15 | [18] |
LM10 | A JCL16 mutant with enhanced ability to utilize amino acids | [3] |
LM11 | LM10 with ΔgdhA, ΔgltB | This study |
LM12 | LM10 with ΔgdhA, ΔgltD | This study |
LM13 | LM10 with ΔglnA, ΔgdhA | This study |
LM14 | LM10 with ΔglnA, ΔgdhA, ΔlsrA | This study |
LM15 | LM14 with plasmids pYX97 and pLM1 | This study |
LM16 | LM14 with plasmids pLM2 and pLM3 | This study |
LM17 | LM14 with plasmids pLM4 and pLM5 | This study |
LM18 | LM14 with plasmids pLM6 and pLM7 | This study |
LM19 | LM14 with plasmids pLM8 and pLM9 | This study |