Fig. 3From: Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sitesSDS-PAGE analysis of purified recombinant enzymes. Lane M, molecular mass markers; lane wt, the wild-type CTendo45; lane 1–7, L47T, Q56T, G63T, R67S, F143T, F143S and W169S; lane 8, T90A; lane 9, the purified CTendo45 treated with PNGase F. a Coomassie blue staining. b Carbohydrate stainingBack to article page