Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites

Table 3 Half-life of CTendo45 and its mutants in thermal inactivation

Substrate	Enzyme	t_1/2 and residual relative activity
Substrate	Enzyme	80 °C	90 °C
CMC-Na	CTendo45	150 min (48.8%)	35 min (52.9%)
	L47T	230 min (53.2%)	55 min (51.3%)
	Q56T	110 min (52.2%)	20 min (46.2%)
	G63T	130 min (48.5%)	25 min (47.5%)
	R67S	190 min (51.7%)	45 min (49.2%)
	T90A	260 min (47.5%)	65 min (52.4%)
	R67S/T90A	320 min (47.8%)	85 min (48.3%)
β-d-glucan	CTendo45	130 min (52.6%)	28 min (49.8%)
	L47T	210 min (53.6%)	45 min (52.3%)
	Q56T	100 min (52.6%)	15 min (54.2%)
	G63T	110 min (53.6%)	20 min (48.6%)
	R67S	160 min (47.1%)	35 min (53.5%)
	T90A	230 min (51.7%)	50 min (49.5%)
	R67S/T90A	270 min (53.0%)	65 min (48.3%)

ISSN: 2731-3654