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Table 6 Strains and plasmids used in this study

From: Coexistence of the Entner–Doudoroff and Embden–Meyerhof–Parnas pathways enhances glucose consumption of ethanol-producing Corynebacterium glutamicum

Strain and plasmid Genotype or description Reference
C. glutamicum
 R Wild-type (JCM 18229) [29]
 CRZ2 R with deletion in ldhA and ppc [21]
 Gly3 CRZ2 with insertion of Ptac-pgi, Ptac-pfkA, Ptac-gapA and Ptac-pyk [12]
 CRZ2e CRZ2 harboring pCRA723 [21]
 CRZ14 Gly3 with insertion of Ptac-tpi [14]
 CRZ14e CRZ14 with overexpression of pdc and adhB from pCRA723 [14]
 CRZ2e-ED CRZ2e with insertion of Ptac-zwf, Ptac-pgl, Ptac-edd-eda This study
 CRZ2e-EDΔpfkA CRZ2e-ED with deletion in pfkA This study
 CRZ14e-ED CRZ14e with insertion of Ptac-zwf, Ptac-pgl, Ptac-edd-eda This study
Plasmid
 pCRA723 Cmr; E. coliCorynebacterium sp. shuttle vector derived from pBL1 with Z. mobilis pdc and adhB [21]