Coexistence of the Entner–Doudoroff and Embden–Meyerhof–Parnas pathways enhances glucose consumption of ethanol-producing Corynebacterium glutamicum

Table 6 Strains and plasmids used in this study

Strain and plasmid	Genotype or description	Reference
C. glutamicum
R	Wild-type (JCM 18229)	[29]
CRZ2	R with deletion in ldhA and ppc	[21]
Gly3	CRZ2 with insertion of Ptac-pgi, Ptac-pfkA, Ptac-gapA and Ptac-pyk	[12]
CRZ2e	CRZ2 harboring pCRA723	[21]
CRZ14	Gly3 with insertion of Ptac-tpi	[14]
CRZ14e	CRZ14 with overexpression of pdc and adhB from pCRA723	[14]
CRZ2e-ED	CRZ2e with insertion of Ptac-zwf, Ptac-pgl, Ptac-edd-eda	This study
CRZ2e-EDΔpfkA	CRZ2e-ED with deletion in pfkA	This study
CRZ14e-ED	CRZ14e with insertion of Ptac-zwf, Ptac-pgl, Ptac-edd-eda	This study
Plasmid
pCRA723	Cm^r; E. coli–Corynebacterium sp. shuttle vector derived from pBL1 with Z. mobilis pdc and adhB	[21]

ISSN: 2731-3654