Fig. 5

Determination of the role of residues S79, S83, S674, and S676 of N. crassa COL-26 in regulation of the dual-affinity glucose transport system. a Subcellular location of WT and point-mutated COL-26. Strains were grown in liquid VMM supplemented with 2% sucrose for 16 h at 28 °C. Scale bar represents 10 µm. b Biomass of different strains grown in liquid VMM containing 2% sucrose for 16 h. c Relative expression levels of glt-1 in different strains in glucose condition. d Relative expression levels of glt-1 in different strains in carbon-free condition. Mycelia were grown in VMM plus 2% sucrose for 16 h, then transferred to VMM with (c) or without (d) 2% glucose. After additional cultivation for 1 h, mycelia were harvested and mRNA was extracted, after which quantitative real-time qPCR was performed to determine glt-1 expression. n.s. not significant; ***P < 0.001