Table 4 Primers used in this study
From: Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae
Primer | Sequence | References or source |
|---|---|---|
BspHI-oriR6K-F | 5′-aatttcatgacagttcaacctgttgatagtac-3′ | [23] |
BsaXI-oriR6K-R | 5′-aattggagaggcggtagagagagacaatgtcagccgttaagtgttc-3′ | [23] |
EcoRI-budCup-F | 5′-aattgaattcagcttccacatctggatcgcccgct-3′ | This study |
EcoRI-budCup-R | 5′-aattgaattcttctctgtccttatagtgagtcaca-3′ | This study |
XbaI-budCdown-F | 5′-aatttctagataaattctaataagctctgacatga-3′ | This study |
SphI-budCdown-R | 5′-aattgcatgccttcatcgtgcgcatttcgcccggc-3′ | This study |
budC-up-F | 5′-aggacatcgtcaataacgacgtgac-3′ | This study |
budC-down-R | 5′-ttcatcttcggtaaagatcagcgtg-3′ | This study |
budC-F | 5′-atgcaaaaagttgctctcgtaaccg-3′ | This study |
budC-R | 5′-ttagttgaacaccatcccaccatca-3′ | This study |
EcoRI-ldhAup-F | 5′ –aattgaattcaccgtgttaagttcaagcgcaccaa-3′ | This study |
BamHI-EcoRI-ldhAup-R | 5′-aattggatccgaattcaagactttctccagtgattttacat-3′ | This study |
XbaI- ldhAdown-F | 5′-aatttctagagccgacatgccgggtggcggttacg-3′ | This study |
SphI-SalI-ldhAdown-R | 5′-aattgcatgcgtcgacggcgacggtcattatttcgcaggcg-3′ | This study |
ldhA-up-F | 5′-tttttggcgcaacggttgacggtgc-3′ | This study |
ldhA-down-R | 5′-atgcgggtcgccgccgcgcctgcca-3′ | This study |
ldhA-F | 5′-atgaaactcgcggtatatagcacaa-3′ | This study |
ldhA-R | 5′-ttagactatctcgttaggacacgct-3′ | This study |