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Table 4 Primers used in this study

From: Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae

Primer Sequence References or source
BspHI-oriR6K-F 5′-aatttcatgacagttcaacctgttgatagtac-3′ [23]
BsaXI-oriR6K-R 5′-aattggagaggcggtagagagagacaatgtcagccgttaagtgttc-3′ [23]
EcoRI-budCup-F 5′-aattgaattcagcttccacatctggatcgcccgct-3′ This study
EcoRI-budCup-R 5′-aattgaattcttctctgtccttatagtgagtcaca-3′ This study
XbaI-budCdown-F 5′-aatttctagataaattctaataagctctgacatga-3′ This study
SphI-budCdown-R 5′-aattgcatgccttcatcgtgcgcatttcgcccggc-3′ This study
budC-up-F 5′-aggacatcgtcaataacgacgtgac-3′ This study
budC-down-R 5′-ttcatcttcggtaaagatcagcgtg-3′ This study
budC-F 5′-atgcaaaaagttgctctcgtaaccg-3′ This study
budC-R 5′-ttagttgaacaccatcccaccatca-3′ This study
EcoRI-ldhAup-F 5′ –aattgaattcaccgtgttaagttcaagcgcaccaa-3′ This study
BamHI-EcoRI-ldhAup-R 5′-aattggatccgaattcaagactttctccagtgattttacat-3′ This study
XbaI- ldhAdown-F 5′-aatttctagagccgacatgccgggtggcggttacg-3′ This study
SphI-SalI-ldhAdown-R 5′-aattgcatgcgtcgacggcgacggtcattatttcgcaggcg-3′ This study
ldhA-up-F 5′-tttttggcgcaacggttgacggtgc-3′ This study
ldhA-down-R 5′-atgcgggtcgccgccgcgcctgcca-3′ This study
ldhA-F 5′-atgaaactcgcggtatatagcacaa-3′ This study
ldhA-R 5′-ttagactatctcgttaggacacgct-3′ This study