Fig. 6

Apparent H₂O₂ production in experiments with LPMO, free copper and 1 mM ascorbic acid or gallic acid. The figure shows hydrogen peroxide accumulation rates obtained in reactions with increasing amounts of LPMO (a) or with 1 µM free copper compared to 1 µM LPMO (b). All experiments were carried out in 50 mM sodium phosphate buffer, pH 6.0, at 30 °C. Reaction mixtures contained 5 U/ml HRP, 100 µM Amplex Red and 2% (v/v) DMSO. Error bars indicate standard deviations between triplicates. Note that plotted values were corrected for resorufin signal repression using H₂O₂ standard curves, obtained in the presence of 1 mM ascorbic acid or gallic acid. The “No LPMO” label indicates that the reaction contained only reductant, with no enzyme added (set up to monitor reactions between reductant and oxygen)