Fig. 5

Analysis of synergistic effect of xylanase on HPAC-pretreated P. densiflora. A Microscope images of the microfibrils of the HPAC-pretreated softwood. CMFs, cellulose microfibrils; arrow, hemicellulose or unspecified material (a scale bar = 3 μm; b scale bar = 400 nm). B Zymogram of the enzymes produced by T. reesei RUT-C30. Two bands representing mannanase activities in enzyme solution is visible on SDS-PAGE gel (arrows). The enzyme was loaded at 6 μg on lane 1 and 3 μg on lane 2, respectively. (C) Synergistic effect of xylanase in the hydrolysis of 1% HPAC-pretreated P. densiflora with 3 FPU for 3 h