Fig. 6

Improvement of the enzymatic hydrolysis rate. a 5% HPAC-pretreated P. densiflora and b 5% Q. acutissima were hydrolyzed with 7.5 and 15 FPU cellulase. The supplementation of accessory enzymes contributed to the remarkable hydrolysis efficiency of the HPAC-pretreated softwood through synergistic effects with 7.5 FPU cellulase. Xyl, xylanase; AA9, auxiliary activity family 9 copper-dependent lytic polysaccharide monooxygenases; Bgl, beta-glucosidase. c Fed-batch enzymatic hydrolysis using 7.5 FPU g biomass⁻¹ and d 15 FPU g biomass⁻¹ with accessory enzymes (Xylanase, AA9, and beta-glucosidase). The initial concentration (5%) was hydrolyzed for 12 h, and additional substrate was added to obtain a total concentration of 10%, as indicated by the arrow. The most economically feasible enzyme dose was found to be 7.5 FPU cellulase at 10% of the substrate when incubated with accessory enzymes