Fig. 5

H₂O₂ accumulation rate (a) and predicted “H₂O₂ consumption rate by LPMOs” (b) during enzymatic saccharification. Reactions containing 5% (w/v) Avicel were initially supplied with 1000 µM ascorbic acid and were run under anaerobic conditions with H₂O₂ feeding. Feed rates used: blue, 50 µmol h⁻¹ H₂O₂; magenta, 100 µmol h⁻¹ H₂O₂; red, 150 µmol h⁻¹ H₂O₂. The rate of H₂O₂ accumulation was determined from a piecewise linear fit of the H₂O₂ concentration (in Fig. 2). The predicted H₂O₂ consumption rate by LPMOs was calculated as the difference of H₂O₂ feed rate and the sum of oxidation rate of AscA and accumulation rate of H₂O₂. The magenta and red arrows indicate the approximate time points where the rate of H₂O₂ accumulation in the medium started increasing for the reactions with 100 and 150 µmol h⁻¹, respectively