Fig. 2
From: Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides
Identification of putative transcriptional regulatory elements. a Distribution of putative fatty acid responsive elements in the upstream sequences of 3 FAD genes. Common DNA motifs were identified using the MEME suite at http://meme-suite.org. E-value is the estimate of the number of motifs expected to find by chance if the letters in the input sequences were shuffled. tss: transcription start site. b Sequence alignment of two candidate OLE1 Regulatory Element (ORE) in the OLE1 promoter. ORE1m is the artificially generated mutant of ORE1, containing 2-nt substitutions. c Luciferase gene reporter assay of hybrid GPD1 promoters. The promoter structures are illustrated on the left, where green, blue and red bars show the location of the inserted DNA motifs. d Effect of OLE1 terminator on gene expression. The Cauliflower mosaic virus 35S gene terminator in POLE1::RtLUC2::T35S was replaced with the 328Â bp terminator of OLE1. The relative promoter activity (RPA) was calculated by normalizing the value against the reading of control construct (POLE1::RtLUC2::T35S) of the same culture conditions and sampling time point